3/29/2023 0 Comments Srdx transcription blocker![]() These results suggested that NGA1 essentially functions as a transcriptional activator of NCED3 among the NGA family proteins. Among the single-knockout mutants of four NGA family genes, we found that the NGATHA1 ( NGA1) knockout mutant was drought-stress-sensitive with a decreased expression level of NCED3 during dehydration stress. The NGA proteins were directly bound to a cis-element NGA-binding element (NBE) in the 5′ untranslated region (5′ UTR) of the NCED3 promoter and were suggested to be transcriptional activators of NCED3. In the present study, we isolated NGATHAs (NGAs) as candidate transcriptional regulators of NCED3 through a screen of a plant library harboring the transcription factors fused to a chimeric repressor domain, SRDX. The enzyme NINE-CIS-EPOXYCAROTENOID DIOXYGENASE 3 (NCED3) is essential for ABA biosynthesis during drought stress, and the NCED3 gene is highly induced by drought stress. Although previous reports revealed detailed molecular roles of ABA in stress responses, the factors that contribute to the drought-stress responses-in particular, regulation of ABA accumulation-remain unclear. The plant hormone abscisic acid (ABA) is accumulated after drought stress and plays critical roles in the responses to drought stress in plants, such as gene regulation, stomatal closure, seed maturation, and dormancy. ![]()
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